Sperm Cryopreservation in Ukraine

Semen cryopreservation (commonly called sperm banking or sperm freezing) is a procedure to preserve sperm cells. Semen can be used successfully indefinitely after cryopreservation. For human sperm, the longest reported successful storage is 24 years. It can be used for sperm donation where the recipient wants the treatment in a different time or place, or as a means of preserving fertility for men undergoing vasectomy or other kinds of treatment that may compromise their fertility, such as chemotherapy, radiation therapy, or surgery.

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Freezing

The most common cryoprotectant used for semen is glycerol (10% in culture medium). Often sucrose or other di-, trisaccharides are added to glycerol solution. Cryoprotectant media may be supplemented with either egg yolk or soy lecithin.
Additional cryoprotectants can be used to increase sperm viability and fertility rates post-freezing. Incorporation of cholesterol into sperm cell membranes with the use of cyclodextrins prior to freezing also increases sperm viability.
Semen is frozen using a newer flash-freezing process known as vitrification. Vitrification gives superior post-thaw motility and cryo survival than slow programmable freezing.

Thawing

Thawing at 40 °C seems to result in optimal sperm motility. On the other hand, the exact thawing temperature seems to have only a minor effect on sperm viability, acrosomal status, ATP content, and DNA.

Why should you choose the ICSI Clinic for IVF in Ukraine?

For 25 years, we have been performing IVF using the ICSI method—the introduction of pre-selected sperm into the egg using a microneedle.

IVF with ICSI is significantly more reliable and effective than standard IVF with spontaneous fertilization of the egg, increasing the chances for everyone, even in difficult cases.

25 years of experience in the field of reproductive technologies. We were one of the first in Ukraine to start treating infertility and have confidently maintained our position as a leader.

Our specialists are true experts in the field of assisted reproductive technologies. They were the first in Ukraine to perform IVF using the ICSI method.

Over a quarter of a century of operation of the clinic, with our help, more than 12,000 babies have been born in 40 countries around the world.

The ICSI Clinic offers various IVF programs; you can choose the best option (different options of IVF with own cells, IVF with DO, IVF with DO and CM, IVF with PGT, IVF with donor sperm).

We offer guaranteed pregnancy programs. This program continues until the baby is born and includes an unlimited number of IVF cycles and procedures.

The clinic is fitted with expert-class equipment.

The success rate of our IVF programs ranges from 70% to 90%.

Remote support of patients during pregnancy resulting from IVF is carried out.

Our own laboratories of cytogenetics and embryology perform a full range of modern research.

Individual selection of an infertility treatment program, assistance to foreign guests of Kyiv in organizing a transfer, selection of housing, and legal support for patients.

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FAQ

How long can frozen sperm be stored?

The shelf life is unlimited.

Does cryopreservation affect the quality of sperm?

No, freezing does not affect the quality, and the use of cryopreservatives protects sperm from possible damage.

At what age is it better to freeze sperm?

According to statistics, sperm from men aged 18-40 have the best characteristics. But it is imperative to take into account the results of the spermogram and individual characteristics.

Is it more difficult to get pregnant with IVF using frozen sperm rather than fresh sperm?

No, the fertilising ability of sperm does not decrease after freezing. According to statistics, the probability of pregnancy in IVF programmes with fresh and frozen sperm is the same.

Can the same sperm sample be frozen twice?

Yes, sometimes it is. But this is done only under certain conditions.