Preimplantation Genetic Testing (PGT) in Ukraine
Preimplantation Genetic Testing (PGT) is the examination of embryos for specific genetic anomalies before their transfer into the uterus during the IVF procedure. The aim of this diagnostic test is to maximize the chances of giving birth to a healthy child for parents at high risk of passing on a hereditary disease. This testing eliminates the probability of transmitting the disease to the child
PGT allows for:
- Detection of aneuploidies – quantitative chromosome anomalies;
- Identification of structural rearrangements;
- Identification of gene mutations.
There are different types:
- Preimplantation Genetic Testing for monogenic diseases;
- Preimplantation Genetic Testing for chromosomal abnormalities;
- Preimplantation Genetic Diagnosis for chromosomal abnormalities.
Preimplantation Genetic Testing for Aneuploidies (PGT-A) is conducted to detect numerical chromosomal anomalies (aneuploidies) in embryos before their transfer to the uterus. This testing aims to increase the success of IVF programs and determine the gender of the future child.
Also conducted is Preimplantation Genetic Testing for Structural Rearrangements (PGT-SR). This analysis is performed under specific indications, particularly when one of the spouses is a carrier of balanced chromosomal rearrangement. The research helps determine the balanced/unbalanced set of chromosomes in the embryo before its implantation into the uterus.
Preimplantation Genetic Testing for Monogenic Diseases (PGT-M) – examination of embryos for specific gene mutations (specific monogenic disease) before their implantation in the uterus. It is used to detect gene mutations in embryos with the aim of maximizing the chances of giving birth to a healthy child. This diagnostic practically eliminates the transmission of the disease to the child and the likelihood of pregnancy termination for medical reasons or the birth of a child with a severe illness.
Before conducting PGT, you will receive comprehensive information during consultations with our specialists – reproductive endocrinologists and geneticists.
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How is Preimplantation Genetic Diagnosis of Gene Mutation (PGT-M) performed
Embryos are obtained using in vitro fertilization. DNA analysis is performed on several cells of the trophectoderm blastocyst. Healthy embryos without pathology are transferred to the uterus.
First in Ukraine:
In Ukraine, the first successful IVF program with PGT due to the risk of X-linked disease (hemophilia) was performed by ISCI Clinic employees, embryologist M.I. Kononenko and geneticist T.E. Zerova-Lyubimova. A healthy child was born in 2003. Years of experience in conducting preimplantation diagnosis have allowed hundreds of children to be born.
Who is recommended for PGT for genetic pathology (PGT-M)
Preimplantation Genetic Testing (PGT-M) will be offered in the presence of the risk of transmitting to the future child:
- Genetic pathology;
- X-linked disease;
- Mitochondrial disease.
PGT for gene mutation is carried out after identifying the mutation gene – DNA analysis of both parents, if necessary, their relatives.
Each specific case of PGT usage is strictly personalized; additional genetic analyses may be proposed to your nearest relatives.
Each specific case of PGT usage is strictly personalized; additional genetic analyses may be proposed to your nearest relatives. Embryo cell biopsy is performed at the blastocyst stage – several cells of the trophectoderm are separated. The biopsy procedure does not harm embryo development. Embryos are frozen until the end of the study. Healthy embryos are transferred in the next cycle.
Genetic diagnosis is performed using next-generation sequencing (NGS) (term of execution – several weeks).
Healthy embryos are transferred into the uterine cavity. Although the accuracy of PGT is extremely high, after pregnancy, we strongly recommend undergoing prenatal fetal diagnosis – this is standard global practice.
Whole-genome screening of embryos:
- The most advanced method;
- Allows studying both genetic diseases and chromosomal ones;
- Provides high reliability – 99.999%;
- Highly sensitive method;
- Analyzes all chromosomes simultaneously;
- Highly automated, minimizing the likelihood of error;
- Detects mosaicism.
Additional Benefits of PGT:
- Reduction of the risk of miscarriage in the first trimester (approximately by 2 times).
- Reduction of the risk of multiple pregnancies.
- Information about the gender of the future child.
- Information about the Rh factor of the future child (in cases of severe Rh conflict in the medical history).
- Detection of hereditary diseases that manifest in adulthood.
Service Costs
| Name of the service | Price |
| Consultation with a geneticist | 1650 UAH |
Preimplantation Genetic Testing specialists
Head of the Laboratory and Laboratory Diagnostic Physician; Geneticist, Ph.D. in Biological Sciences
Preimplantation Genetic Testing for Chromosomal Abnormalities
Preimplantation genetic testing distinguishes between preimplantation genetic testing for aneuploidy (PGT-A) and preimplantation genetic testing for structural chromosome rearrangements (PGT-SR).
Preimplantation Genetic Testing for Aneuploidy (PGT-A)
PGT-A is conducted to detect quantitative chromosomal anomalies in embryos before their transfer to the uterus. PGT-A increases the success rate of IVF programs, determines the gender of the future child, and reduces the risk of miscarriage in the first trimester by half.
Cases Where PGT-A is Conducted
Preimplantation genetic testing for aneuploidy is conducted under the following indications:
- Women over 35 years of age.
- History of miscarriage.
- Failure of embryo implantation in several IVF cycles.
How PGT-A is Conducted
Embryos are obtained through in vitro fertilization (IVF). Diagnosis is performed on several cells of the blastocyst trophectoderm. Healthy embryos, without chromosomal abnormalities, are transferred to the uterus.
Years of experience in preimplantation diagnosis have allowed hundreds of children to be born.
Who is Recommended PGT-A
PGT-A is recommended in cases of:
- Multiple spontaneous miscarriages, biochemical pregnancies, or unsuccessful IVF cycles.
- Infertility of unclear origin.
- Male factor infertility.
- Desire to maximize the chance of having a child without genetic pathology.
Before Conducting PGT-A
Before conducting PGT-A, you will receive comprehensive information after consulting with our specialists – reproductive endocrinologist and geneticist.
The IVF program includes ovarian stimulation and sperm fertilization using ICSI.
Embryo cell biopsy is performed at the blastocyst stage – several cells of the trophectoderm are separated. The biopsy procedure does not harm the embryo’s development. Embryos are frozen until the end of the study. Healthy embryos are transferred in the next cycle.
Depending on the specific situation, our clinic uses: – Fluorescence in situ hybridization (FISH). – Next-generation sequencing (NGS).
Embryos without chromosomal abnormalities are transferred to the uterine cavity. Although the accuracy of genetic testing (screening) is very high, after pregnancy occurs, we strongly recommend prenatal fetal diagnosis – standard global practice.
Fluorescence In Situ Hybridization (FISH)
Historically, the first method of diagnosing chromosomal abnormalities at the preimplantation level allows simultaneous investigation of the presence/absence of several chromosomes in the embryo cell(s). The analysis takes from 2-3 days to several weeks.
Next-Generation Sequencing (NGS)
NGS is a molecular DNA analysis that identifies genetic damage (mutations) in DNA, examining all 23 pairs of chromosomes.
Whole-Genome Screening of Embryos
The most advanced method allows investigation of both genetic diseases and chromosomal abnormalities, providing high accuracy (99.999%), maximum sensitivity, simultaneous analysis of all chromosomes, highly automated to minimize the probability of error, and detects mosaicism.
Advantages of PGT-A
PGT-A allows:
- Prevention of the birth of a child with pathology.
- Increased success of the IVF program.
- Reduced risk of pregnancy loss (approximately by half).
- Reduced risk of multiple pregnancies.
- Knowledge of the gender of the future child.
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Preimplantation Genetic Testing for Structural Chromosomal Rearrangements (PGT-SR)
When PGT-SR is Conducted
Preimplantation genetic testing for structural rearrangements is conducted under the following indications:
- Presence of a structural rearrangement in one of the parents. History of pregnancy loss.
- History of pregnancy loss.
- Failure of embryo implantation in several IVF cycles.
How PGT-SR is Conducted
Embryos are obtained through in vitro fertilization (IVF). Diagnosis is performed on several cells of the blastocyst trophectoderm. Healthy embryos are then transferred to the uterus.
Years of experience in preimplantation diagnosis have allowed hundreds of children to be born.
Who is Recommended PGT-SR
PGT-SR is recommended if there is a history of:
- Multiple spontaneous miscarriages, biochemical pregnancies, or unsuccessful IVF cycles.
- Infertility of unclear origin.
- Male factor infertility.
Before Conducting PGT-SR
Before conducting PGT-SR, you will receive comprehensive information after consulting with our specialists – reproductive endocrinologist and geneticist.
The IVF program includes ovarian stimulation and sperm fertilization using ICSI.
Embryo cell biopsy is performed at the blastocyst stage – several cells of the trophectoderm are separated. The biopsy procedure does not harm the embryo’s development. Embryos are frozen until the end of the study. Healthy embryos are transferred in the next cycle.
Depending on the specific situation, our clinic uses: – Fluorescence in situ hybridization (FISH). – Next-generation sequencing (NGS).
Embryos without chromosomal abnormalities are transferred to the uterine cavity. Although the accuracy of genetic testing (screening) is very high, after pregnancy occurs, we strongly recommend prenatal fetal diagnosis – standard global practice.
Fluorescence In Situ Hybridization (FISH)
Historically, the first method of diagnosing chromosomal abnormalities at the preimplantation level allows simultaneous investigation of the presence/absence of several chromosomes in the embryo cell(s). The analysis takes from 2-3 days to several weeks.
Next-Generation Sequencing (NGS)
Whole-genome screening of embryos: the most advanced method allows investigation of both genetic diseases and chromosomal abnormalities, providing high accuracy (99.999%), maximum sensitivity, simultaneous analysis of all chromosomes, highly automated to minimize the probability of error, and detects mosaicism.
Advantages of PGT-SR
In addition to excluding the birth of a child with chromosomal pathology, PGT-SR allows:
- Increased success of the IVF program.
- Reduced risk of miscarriage in the first trimester (approximately by half).
- Decreased risk of multiple pregnancies.
Sex selection
Sex selection within the IVF program plus PGT-A. Some genetic diseases are associated with the X chromosome (X-linked genetic diseases). Among them: hemophilia A (classic hemophilia), color blindness, X-linked ichthyosis, Duchenne muscular dystrophy, and others.
X-linked diseases are recessive in inheritance type and follow these rules:
- The disease is never passed from father to son.
- The disease predominantly affects males.
- The trait (disease) can be transmitted through phenotypically healthy daughters of an affected father to 50% of his grandsons.
- Carriers may sometimes exhibit subclinical signs of the pathology.
Before conducting PGT for X-linked diseases, you will receive comprehensive information after consultation with our specialists – reproductive endocrinologist and geneticist.
Sometimes sex selection is performed for social reasons.
Why should you choose the ICSI Clinic for IVF in Ukraine?
25 years of experience in the field of reproductive technologies. We were one of the first in Ukraine to start treating infertility and have confidently maintained our position as a leader.
Over a quarter of a century of operation of the clinic, with our help, more than 12,000 babies have been born in 40 countries around the world.
We offer guaranteed pregnancy programs. This program continues until the baby is born and includes an unlimited number of IVF cycles and procedures.
The success rate of our IVF programs ranges from 70% to 90%.
Our own laboratories of cytogenetics and embryology perform a full range of modern research.
Individual selection of an infertility treatment program, assistance to foreign guests of Kyiv in organizing a transfer, selection of housing, and legal support for patients.
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